SP6 RNA polymerase
Fornecedor: Promega
SP6 RNA Polymerase is a DNA-dependent RNA polymerase used for in vitro transcription. Only SP6 DNA or DNA cloned downstream from an SP6 promoter can be used as a template for SP6 RNA Polymerase-directed RNA synthesis. The polymerase can incorporate ³²P, ³³P, ³H and ³⁵S nucleoside triphosphates.
- Exhibits extremely high affinity and specificity for SP6 promoter sequences
- >90% pure as determined by SDS polyacrylamide gel electrophoresis
- Free of detectable levels of contaminating RNase and DNase activity (<1% release)
- Source: Recombinant E. coli strain
Applications:
Generation of templates for in vitro translation
Generation of probes for nucleic acid hybridisations
Generation of RNA processing substrates
Generation of antisense RNA
Storage buffer: 20 mM potassium phosphate buffer (pH 7,7 at 25 °C), 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0,1% (v/v) Triton® X-100 and 50% (v/v) glycerol.
Unit definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 5 nmol of CTP into acid-insoluble product in 60 minutes at 37 °C in a total volume of 100 μl.
Fornecimento: Kit includes SP6 RNA Polymerase and transcription-optimised 5X Buffer (200 mM Tris-HCl, pH 7,9 at 25 °C; 30 mM MgCl₂; 10 mM spermidine; 50 mM NaCl).
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