T3 RNA polymerase
Fornecedor: Promega
T3 RNA Polymerase is a DNA-dependent RNA polymerase that synthesises RNA using only T3 DNA or DNA templates cloned downstream from a T3 promoter. The polymerase can incorporate ³²P, ³³P, ³H and ³⁵S nucleoside triphosphates during transcription.
- Exhibits extremely high affinity and specificity for T3 promoter sequences
- >90% pure as determined by SDS polyacrylamide gel electrophoresis
- Free of detectable levels of contaminating RNase and DNase activity (<1% release)
- Source: Recombinant E. coli strain
Applications:
Generation of templates for in vitro translation
Generation of probes for nucleic acid hybridsations
Generation of RNA processing substrates
Generation of antisense RNA
Storage buffer: 20 mM potassium phosphate (pH 7,7 at 25 °C), 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0,1% (v/v) Triton® X-100 and 50% (v/v) glycerol.
Unit definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 5 nmol of CTP into acid-insoluble product in 60 minutes at 37 °C in a total volume of 100 μl.
Fornecimento: Supplied with 100 mM DTT and transcription optimised 5X buffer: 200 mM Tris-HCl (pH 7,9 at 25 °C), 30 mM MgCl₂, 10 mM spermidine, 50 mM NaCl.
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