Calpain-Glo™ Protease Assay
Fornecedor: Promega
The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (µ) and 2 (m) activities.
- Designed for measuring calpain activity and/or inhibition in a multiwell format and easy to automate
- Maximum sensitivity is reached in as little as 10 minutes, while the enzyme is fully active
- Up to 1000 times more sensitive than competitive fluorometric assays
- Assay is linear over 4 logs of calpain concentration
- Luminescent assay avoids inherent fluorescent background signals, providing excellent signal-to-background ratio
The Calpain-Glo™ Protease Assay provides a succinyl, proluminescent calpain substrate, Suc-LLVY-aminoluciferin, in a buffer system optimised for calpain and luciferase activities. The addition of the calpain reagent in an "add-mix-measure" format results in calpain cleavage of the substrate and rapid development of a "glow-type" luminescent signal produced by the luciferase reaction. The signal is proportional to the calpain activity. Designed for use with purified enzyme preparations.
Fornecimento: The complete assay kit includes Calpain-Glo™ buffer, Suc-LLVY-Glo™ substrate and luciferin detection reagent.
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