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Calpain-Glo™ Protease Assay

Fornecedor: Promega

G8501 G8502
PROMG8501EA 690 EUR
PROMG8501 PROMG8502
Calpain-Glo™ Protease Assay
Ensaios Ensaios celulares

The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (µ) and 2 (m) activities.


  • Designed for measuring calpain activity and/or inhibition in a multiwell format and easy to automate
  • Maximum sensitivity is reached in as little as 10 minutes, while the enzyme is fully active
  • Up to 1000 times more sensitive than competitive fluorometric assays
  • Assay is linear over 4 logs of calpain concentration
  • Luminescent assay avoids inherent fluorescent background signals, providing excellent signal-to-background ratio


The Calpain-Glo™ Protease Assay provides a succinyl, proluminescent calpain substrate, Suc-LLVY-aminoluciferin, in a buffer system optimised for calpain and luciferase activities. The addition of the calpain reagent in an "add-mix-measure" format results in calpain cleavage of the substrate and rapid development of a "glow-type" luminescent signal produced by the luciferase reaction. The signal is proportional to the calpain activity. Designed for use with purified enzyme preparations.


Fornecimento: The complete assay kit includes Calpain-Glo™ buffer, Suc-LLVY-Glo™ substrate and luciferin detection reagent.

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