Apoptosis assays, Caspase-Glo® 8 Assay
Fornecedor: Promega
The Caspase-Glo® 8 Assay is a homogeneous luminescent assay that measures caspase-8 activity. The assay provides a proluminescent caspase-8 substrate in a buffer system optimised for caspase-8 activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo® 8 reagent results in cell lysis, followed by caspase cleavage of the substrate and generation of "glow-type" luminescent signal. The signal is proportional to caspase-8 activity.
- Add-mix-measure protocol amenable to automation
- Low background luminescence results in excellent signal-to-noise ratios, allows assay to be performed in 96- or 384-well formats
- No sample preparation, manipulation and extended incubation times required, maximum sensitivity can be achieved in as little as 30 minutes to 1 hour
- Delivers excellent Z´ factor values in cell and purified enzyme models
Fornecimento: The complete assay kit includes Caspase-Glo® 8 buffer, Caspase-Glo® 8 substrate and MG-132 inhibitor.
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