CYP1A2 assay and screening systems, P450-Glo™
Fornecedor: Promega
The P450-Glo CYP1A2 assays provide a homogeneous, luminescent method for measuring cytochrome CYP1A2 activity.
- Broad dynamic range, low background and better sensitivity for less ambiguous data
- Luminescent output eliminates interference from fluorescent test compounds
- Optimal data quality for microplate reader assay
- Simplified, homogeneous experimental protocol
The P450-Glo CYP1A2 screening system provides a complete set of reagents for performing luminescent cytochrome P450 assays. The membranes are prepared from baculovirus-infected insect cells and contain human cytochrome P450, P450 reductase, and cytochrome b5. It also contains a membrane fraction devoid of cytochrome P450 activity as a negative control. The assays are ideal for testing the effects of drugs and new chemical entities on cytochrome P450 enzyme activities.
Fornecimento: Assay system contains Luciferin-ME, 5 mM (1×200 µl or 1×1 ml); luciferin detection reagent (lyophilised) (1 vial); reconstitution buffer (1×10 or 1×50 ml). Screening system contains P450-Glo CYP1A2 assay includes Luciferin-ME, 5 mM (1 ml); luciferin detection reagent (lyophilised) (1 vial); reconstitution buffer (50 ml) and human CYP1A2 enzyme system includes CYP1A2 (1 pmol/µl) and reductase (500 µl); control membranes (100 µl); potassium phosphate buffer, 1M (pH 7.4) (5 ml); luciferin-free water (50 ml); NADPH regeneration system solution A (2,75 ml); NADPH regeneration system solution B (0,6 ml).
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