Cytotoxicity assays CytoTox-Fluor™
Fornecedor: Promega
The CytoTox-Fluor™ Cytotoxicity assay is a single reagent addition, homogeneous, fluorescent assay that measures the relative number of dead cells in cell populations. The assay measures a distinct protease activity associated with cytotoxicity and uses a fluorogenic peptide substrate (bis-alanyl-alanyl-phenylanlanyl-rhodamine 110; bis-AAF-R110) to measure dead-cell activity, which has been released from cells that have lost membrane integrity.
- Eliminates parallel plate processing and reduces cell culture costs
- Designed to accommodate downstream multiplexing with several cell based assays
- Data normalisation for dead cell number makes results more comparable well-to-well, plate-to-plate, day-to-day
- Reduced assay variability – homogeneous 'add-mix-measure' protocol avoids the cumulative error associated with multistep protocols
Fornecimento: The complete assay kit includes bis-AAF-R110 substrate and assay buffer.
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