Vectors, HQ and GST Tag Flexi®
Fornecedor: Promega
Flexi® vectors are used for inducible expression of HQ- and GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.
- Easy to implement and reliable
- Reusable and cost-efficient Ni (His-affinity) and gluthathione (GST-affinity) resins
It is based on two rare-cutting restriction enzymes, SgfI and PmeI, to provide an efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi vectors without needing to resequence. All Flexi vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for successful ligation of the insert.
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