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Vectors, HQ and GST Tag Flexi®

Fornecedor: Promega

C8441 C8451 C8471 C8461
PROMC8441EA 548 EUR
PROMC8441 PROMC8451 PROMC8471 PROMC8461
Vectors, HQ and GST Tag Flexi®
Vectors, Plasmids and Libraries

Flexi® vectors are used for inducible expression of HQ- and GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.


  • Easy to implement and reliable
  • Reusable and cost-efficient Ni (His-affinity) and gluthathione (GST-affinity) resins


It is based on two rare-cutting restriction enzymes, SgfI and PmeI, to provide an efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi vectors without needing to resequence. All Flexi vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for successful ligation of the insert.

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