Procurou por: Western Blotting Reagents

Fornecedor: Thermo Fisher Scientific

Descrição: These kits include SuperSignal™ West Pico Substrate that is a highly sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. The kits also contain reagents for the specific detection of biotinylated antibodies, rabbit IgG or mouse IgG. The substrate’s intense signal output enables detection of picogram amounts of antigen. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.

Fornecedor: Thermo Fisher Scientific

Descrição: OPD (o-phenylenediamine dihydrochloride) is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492 nm by ELISA plate readers.

Código de Artigo: (PIER20158)

Fornecedor: Thermo Fisher Scientific

Descrição: LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

UOM: 1 * 1 unid.

Promoção

Fornecedor: Thermo Fisher Scientific

Descrição: The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Fornecedor: Rockland Immunochemicals

Descrição: TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

Fornecedor: Rockland Immunochemicals

Descrição: FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Fornecedor: Thermo Fisher Scientific

Descrição: SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

MSDS Certificados

Fornecedor: Thermo Fisher Scientific

Descrição: The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Código de Artigo: (WB59-100)

Fornecedor: Merck Millipore (Oncogene)

Descrição: This Western-Re-Probe™ reagent is validated for use in immunoblotting.

UOM: 1 * 100 mL

Promoção

Fornecedor: G-Biosciences

Descrição: Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Fornecedor: Thermo Fisher Scientific

Descrição: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Código de Artigo: (J63209.AK)

Fornecedor: Thermo Fisher Scientific

Descrição: Saponin permeating solution, is used as a cell permeabilising agent.

UOM: 1 * 250 mL

MSDS Certificados Promoção

Fornecedor: Thermo Fisher Scientific

Descrição: For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

MSDS Certificados

Código de Artigo: (PIER15159)

Fornecedor: Thermo Fisher Scientific

Descrição: QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.

UOM: 1 * 1 unid.

Promoção

Fornecedor: Cytiva

Descrição: AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

Fornecedor: Cytiva

Descrição: The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.