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CYP2D6 assay and screening systems, P450-Glo™

Fornecedor: Promega

V8891 V8892 V9890
PROMV8891EA 243 EUR
PROMV8891 PROMV8892 PROMV9890
CYP2D6 assay and screening systems, P450-Glo™
Ensaios Ensaios celulares

The P450-Glo CYP2D6 assays provide a homogeneous, luminescent method for measuring cytochrome CYP2D6 activity.


  • Broad dynamic range, low background and better sensitivity for less ambiguous data
  • Luminescent output eliminates interference from fluorescent test compounds
  • Optimal data quality for microplate reader assay
  • Simplified, homogeneous experimental protocol


The P450-Glo CYP2D6 screening system provides a complete set of reagents for performing luminescent cytochrome P450 assays. The membranes are prepared from baculovirus-infected insect cells and contain human cytochrome P450, P450 reductase, and cytochrome b5. It also contains a membrane fraction devoid of cytochrome P450 activity as a negative control. The assays are ideal for testing the effects of drugs and new chemical entities on cytochrome P450 enzyme activities.


Fornecimento: Assay system contains Luciferin-ME EGE (1×240 or 1×900 µg); luciferin detection reagent (lyophilised) (1 vial); reconstitution buffer with esterase (1×10 or 1×50 ml). Screening system contains P450-Glo CYP2D6 assay includes Luciferin-ME EGE (900 µg); luciferin detection reagent (lyophilised) (1 vial); reconstitution buffer with esterase (50 ml) and human CYP2D6 enzyme system includes CYP2D6 (1 pmol/µl), reductase and b5 (250 µl); control membranes (100 µl); potassium phosphate buffer, 1M (pH 7.4) (5 ml); luciferin-free water (50 ml); NADPH regeneration system solution A (2,75 ml); NADPH regeneration system solution B (0,6 ml).

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