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Vector systems, pGEM®-T and pGEM®-T Easy

Fornecedor: Promega

A1360 A1380 A3600 A3610
PROMA13600020EA 230 EUR
PROMA13600020 PROMA13800020 PROMA36000020 PROMA36100020
Vector systems, pGEM®-T and pGEM®-T Easy
Vectors, Plasmids and Libraries

The pGEM®-T and pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments.


  • Flexibility: Insert excision with a either BstZI single digest (pGEM®-T) or BstZI, EcoRI or NotI single digest (pGEM®-T Easy)
  • Rapid Ligation Buffer (2X) allows reactions to be completed in one hour at room temperature
  • Available with or without competent cells


The pGEM®-T Vector is ready to use in ligation reactions, prepared by cutting the pGEM®-5Zf(+) Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid by preventing recircularisation of the vector and providing a compatible overhang for ligation of PCR products with A overhangs.


The pGEM®-T Easy Vector Systems offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for BstZI, EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.


T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by Blue/White Screening on indicator plates. The vector allows preparation of single-stranded DNA due to its f1 Origin of Replication.


Fornecimento: System includes pGEM®-T ir pGEM®-T easy vector (1×1,2 µg, 50 ng/µl), rapid ligation Buffer (2X), control insert DNA and T4 DNA ligase. The pGEM®-T vector system II and pGEM®-T easy vector system II contain JM109 competent cells in addition to all system I components.

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